Everything You Need to Know About Ion exchange chromatography
-
Loading... - Grace Poku
- 18 Apr 2023
- 67 Views
- 0 Like
- 0 Comment
The separation of ionizable molecules according to their aggregate charges is the goal of the chromatographic technique known as ion exchange chromatography. Because the charge carried by the molecule of interest can be easily changed by changing the buffer pH, this technique permits the separation of comparable types of molecules that would be difficult to separate using other techniques. This would be the case if other techniques were used.
Ion exchange chromatography is frequently utilised for the separation of inorganic anions (such as chloride, nitrate, and sulphate), as well as inorganic cations (e.g., lithium, sodium, and potassium). It is also possible to utilise it for organic ions, although this use is becoming less prevalent as a result of the development of technologies for reversed phase liquid chromatography, which will be discussed further on. Ion-exchange chromatography is used as a stage in the process of purifying proteins, which is another notable application of this technique. Because some of the substituent groups of amino acids are charged (the total charge for a given protein is a function of the pH of the solution), ion exchange is an effective technique for purifying proteins. This is because the total charge of a protein is dependent on the pH of the solution.
Ion exchange chromatography Vs Ion Chromatography
Ions in the sample can be “paired” and separated as the ion pair in ion pair chromatography, whereas in ion exchange chromatography, ions in the sample can be separated as cations and anions separately. This is the primary distinction between ion pair chromatography and ion exchange chromatography. Ion pair chromatography is more commonly used.
Chromatography is an important technology that may separate various components that are present in a mixture. We can use analytical techniques such as ion pair and ion exchange chromatography to separate ions and polar molecules in a mixture depending on the electrical charge that they carry with them. These techniques are called ion pair chromatography and ion exchange chromatography, respectively. Read More…
